Synchrotron SAXS
data from solutions of
ACTN2-T247M using SEC-SAXS (aggregate peak)
in
20 mM HEPES, 150 mM NaCl, pH 8
were collected
on the
B21 beam line
at the Diamond Light Source storage ring
(Didcot, UK)
using a Eiger 4M detector
at a sample-detector distance of 3.7 m and
at a wavelength of λ = 0.94 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
One solute concentration of 5.00 mg/ml was measured
at 4°C.
600 successive
3.200 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
ACTN2 with T247M missense variant using SEC-SAXS with aggregate peak selected
s, nm-1
