Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)

Adriana Erica Miele.

SASDZK8 – Schistosoma mansoni Venom Allergen-like protein isoform 13, alone

Venom allergen-like protein 13
MWexperimental 22 kDa
MWexpected 27 kDa
VPorod 32 nm3
log I(s) 1.60×102 1.60×101 1.60×100 1.60×10-1
Venom allergen-like protein 13 small angle scattering data  s, nm-1
ln I(s)
Venom allergen-like protein 13 Guinier plot ln 1.61×102 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Venom allergen-like protein 13 Kratky plot 1.104 0 √3 sRg
p(r)
Venom allergen-like protein 13 pair distance distribution function Rg: 3.1 nm 0 Dmax: 15.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Synchrotron SAXS data from solutions of Schistosoma mansoni Venom Allergen-like protein isoform 13, alone in 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH 6.8 were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus3 2M detector at a sample-detector distance of 2.8 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.8 and 7.3 mg/ml were measured at 20°C. One 1 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.

Venom allergen-like protein 13 (SmVAL13)
Mol. type   Protein
Organism   Schistosoma mansoni
Olig. state   Monomer
Mon. MW   27.2 kDa
 
UniProt   Q1WIQ5 (1-236)
Sequence   FASTA