Synchrotron SAXS
data from solutions of
Schistosoma mansoni Venom Allergen-like protein isoform 13 (SmVAL13) in complex with pAb anti-SmVAL13 in ratio 0.5:1 (2nd peak)
in
25 mM MES, 125 mM NaCl, 1 mM EDTA, pH 6.8
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a Eiger 4M detector
at a sample-detector distance of 2 m and
at a wavelength of λ = 0.76 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample
at 3 mg/ml was injected at a 0.30 ml/min flow rate
onto a Cytiva Superdex 200 Increase 3.2/300 column
at 20°C.
2600 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SmVAL13 in complex with its cognate pAb at a 0.5:1 ratio was analyzed by SEC-SAXS. Spectral deconvolution was performed using Evolving Factor Analysis (EFA), revealing two distinct peaks. However, only one peak could be reliably analyzed due to differences in scattering intensity. The present analysis focuses on the second peak.
s, nm-1
