Schistosoma mansoni Venom Allergen-like protein 13 (SmVAL13) in complex with cognate polyclonal antibody (pAb anti-SmVAL13)

Adriana Erica Miele.

SASDZR8 – Polyclonal antibody anti-SmVAL13

Polyclonal antibody anti-SmVAL13
MWexperimental 147 kDa
MWexpected 140 kDa
VPorod 214 nm3
log I(s) 5.51×10-1 5.51×10-2 5.51×10-3 5.51×10-4
Polyclonal antibody anti-SmVAL13 small angle scattering data  s, nm-1
ln I(s)
Polyclonal antibody anti-SmVAL13 Guinier plot ln 5.52×10-1 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
Polyclonal antibody anti-SmVAL13 Kratky plot 1.104 0 √3 sRg
p(r)
Polyclonal antibody anti-SmVAL13 pair distance distribution function Rg: 5.1 nm 0 Dmax: 16.2 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Polyclonal antibody anti-SmVAL13 DAMMIF model
Synchrotron SAXS data from solutions of Polyclonal antibody anti-SmVAL13 in 25 mM MES, 125 mM NaCl, 1 mM EDTA, pH 6.8 were collected on the SWING beam line at the SOLEIL storage ring (Saint-Aubin, France) using a Eiger 4M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.76 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 45.00 μl sample at 3.3 mg/ml was injected at a 0.30 ml/min flow rate onto a column at 20°C. 2600 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Polyclonal antibody anti-SmVAL13 alone analyzed by SEC-SAXS.

Polyclonal antibody anti-SmVAL13 (pAb anti-SmVAL13)
Mol. type   Protein
Organism   Lagomorph
Olig. state   Monomer
Mon. MW   140 kDa
Sequence   FASTA