| MWexperimental | 43 | kDa |
| MWexpected | 44 | kDa |
| VPorod | 55 | nm3 |
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log I(s)
1.11×101
1.11×100
1.11×10-1
1.11×10-2
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s, nm-1
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Freezing the P-Rex2 DH/PH tandem
Lauren Anderson.SASDZT5 – Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 DH/PH tandem|
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Fits and models
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Synchrotron SAXS
data from solutions of
Frozen human P-Rex2 DH/PH (Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein) without cryoprotectant
in
20 mM HEPES, 200 mM NaCl, 2 mM DTT, 1% glycerol, pH 7
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a sample-detector distance of 2.1 m and
at a wavelength of λ = 0.0127 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 2 mg/ml was injected at a 0.65 ml/min flow rate
onto a Shodex KW-800 series column
.
750 successive
2 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. Sample injection volume = UNKNOWN |
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