Pikachurin

Pika-LG3 protein

Organism: Homo sapiens

Monomeric molecular weight: 22.0 kDa

Oligomeric state: Monomer

Total molecular weight: 22.0 kDa

UniProt: Q63HQ2 (831-1017)

Sequence:

GSHHHHHHGSAIIEAIEIPQFIGRSYLTYDNPDILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAKAAA

Recombinant N-terminal His-tagged Pika-LG3 was expressed using HEK293-F cells. The sample was purified using IMAC, followed by gel filtration in 25 mM HEPES/NaOH, 200 mM NaCl, pH 8.