Polyubiquitin-B

HOTag6-G10-Ub protein

Organism: Homo sapiens

Monomeric molecular weight: 13.2 kDa

Oligomeric state: Tetramer

Total molecular weight: 53.0 kDa

UniProt: P0CG47 (1-76)

Sequence:

MTLREIEELLRKIIEDSVRSVA ELEDIEKWLKKIGGGGGGGGGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

HOTag6 is a ~30-amino-acid tetramerization domain followed by a 10-Gly residue linker and then the sequence for monoubiquitin. This is a designed protein (no natural variant exists). The HOTag6 sequence is from the Shu group at UCSF (DOI: 10.1016/j.molcel.2017.12.008).