Metacaspase-1

CgMCA I protein

Organism: Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)

Monomeric molecular weight: 46.0 kDa

Oligomeric state: Monomer

Total molecular weight: 46.0 kDa

UniProt: Q6FPX9 (1-392)

Sequence:

MGSSHHHHHHSSGLVPRGSHMYPGSGNYSYNNRPSMPPPGFNGDGQGYRQEYGNQYGGGY QQQQYQDQYQGENRGQYQGQYQDQPEYGRPPSGMVRPPSSIQQGNGQQFQYSQMTGRRKA LLIGINYIGSKNALRGCINDAHNIFNYLTTYCGYRPEDIVMLTDDQREMVKIPLKENIIR AMQWLVKDAQPNDALFFHYSGHGGQTKDLDGDEEDGMDDVIYPVDFESVGPLIDDTMHDI MVKSLPQGARLTALFDSCHSGTVLDLPYTYSTKGVIKEPNMWKDVGSDGIQAAMAYATGN RSALFSSIGNMVSSVTKKQNVDRERVRQIKFSPADVIMLSGSKDNQTSADTFADGQNIGA MSHAFISVMTRQPQQSYLSLLQNLRNELAGKYSQKPQLSASHPIDVNLQFIM

Metacaspases are caspase-like homologs which undergo a complex maturation process involving multiple intra-chain cleavages resulting in a composite enzyme made of a p10 and a p20 domain. The molecule used in the experiment is the mature form. The construct contains a N-terminal MGSSHHHHHHSSGLVPRGSH tag.