Serine/arginine (SR)-type shuttling mRNA binding protein NPL3

Npl3 (P196D; A197D) protein

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Monomeric molecular weight: 18.3 kDa

Oligomeric state: Monomer

Total molecular weight: 18.3 kDa

UniProt: Q01560 (120-280)

Sequence:

GELSNTRLFVRPFPLDVQESELNEIFGPFGPMKEVKILNGFAFVEFEEAESAAKAIEEVHG KSFANQPLEVVYSKLDDKRYRITMKNLPEGCSWQDLKDLARENSLETTFSSVNTRDFDGT GALEFPSEEILVEALERLNNIEFRGSVITVERDDNPPPIR

Yeast Npl3 is a serine/arginine-rich mRNA binding protein comprises two tandem RRMs (RNA recognition motifs). SAXS measurement was performed with the 120-280 amino acid construct of Npl3 which comprises tandem RRMs where the linker connecting to RRM1 and RRM2 is mutated at P196D & A197D positions.