Dual specificity mitogen-activated protein kinase kinase 6

MKK6DDGRA protein

Organism: synthetic construct

Monomeric molecular weight: 41.8 kDa

Oligomeric state: Monomer

Total molecular weight: 41.8 kDa

Sequence:

MGSQLLERRGVSELPPLYIPKEAFEQPQTSSTPPRDLDSKACISIGNQNFEVKADDLEPIMELGRGAYGVVEKMRHVPSGQIMAVKRIRATVNSQEQKRLLMDLDISMRTVDCPFTVTFYGALFREGDVWICMELMDTSLDKFYKQVIDKGQTIPEDILGKIAVSIVKALEHLHSKLSVIHRDVKPSNVLINALGQVKMCDFGISGYLVDDVAKDIDAGCKPYMAPERINPELNQKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGDLEVLFQGPWSHPQFEKGGGSGGGSGGSAWSHPQFEK

To stabilize this complex for structural studies, a chimera of MKK6, named MKK6DDGRA, was created where the native KIM domain was replaced with that from the Toxoplasma gondii effector protein GRA24.