Acylamino-acid-releasing enzyme (I277L, V491A)

S9Cgs protein

Organism: Geobacillus stearothermophilus

Monomeric molecular weight: 77.1 kDa

Oligomeric state: Tetramer

Total molecular weight: 308.4 kDa

UniProt: A0A150M835 (17-670)

Sequence:

MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGTDLLRLRSVRDPHYAPDGTRAVFVEKSIDEEKQYRSHLWIWAADGSVRQWTFGRWRDMKPRFSPRGEIIAFLSDRSGRTQLWLLPANGGEARQLTFFKNGVRDYVWSPDGTFLITLTTLGDDETIEDREEPEKVEVKRADLKPRVVERLYYKSDASGFLDGKRAVLTRIDVLSGKSEALTGREEEIGSFAISPNGRTLAFVANRNEDPDTTFTRDIVLLDLESKAETNLTNGCGTFASLAWSPDGTKLAAIGHDLAYLGATLHRLYVFEPERGTKRVLTADWDVHLGDAMVGDTHADAKGPGPIWASDGSGLYVTASERGRVNLYFVSLAGPIVPVIEGNFHLYGLAIHPSEQQAIAAISSPTSVGDLYAVSLADGTKTRLTRANEALENEVVFADAEPFTYRSADGLEIQGWIMKPPELDEGEKAPLVVEIHGGPHAMYGFTFFHELQLLASSGYAVLFTNPRGSHGYGQSFVNAVRGDYGGMDYEDIMAGVDAAISKFDFIDKERLGVTGGSYGGFMTNWIVGHTDRFKAAVTQRSISNWLSFSGVSDIGYFFTKWEVGCDVWEDAERLWHHSPLKYVKHMRTPLLILHSERDYRCPIEQAEQLFVALKQLGRETKLVRFPDANHDLSRTGNPALRLERLRHIVDWFDRYLKG

Acylamino-acid-releasing enzyme containing the amino acid mutations I277L, V491A. The protein construct used for SAXS contains an additional non-native polyhistidine extension and amino acid linker sequence at the N-terminus (MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGT).