Probable chromosome-partitioning protein ParB (P235G, D237G, K239A)

vcParB1-linker protein

Organism: Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Monomeric molecular weight: 34.1 kDa

Oligomeric state: Dimer

Total molecular weight: 68.2 kDa

UniProt: Q9KNG7 (2-293)

Sequence:

MGMHHHHHHENLYFQGTKRGLGKGLDALLATSSLAREKQQVASLSQSMSAEGELADLSISNLKPGIYQPRKDLSPEALEELAASIQSQGIIQPIVVRHLPTGGYEIIAGERRWRAAKQAGLKQVPCLIKQVEDRGAIAMALIENIQREDLNAMEEAQALERLQNEFNLTHQQVAEVIGKSRTTVTNLLRLNQLSDDVKRLLETKQLEMGHARALLMLEGEQQVEIAQQVAKKQLTVRQTEQLVKKCLSDGSGAANVSEDLEIQQLSQNLSEKLAAKVSIVRTPNGKSKVTISLDEPHKLELLIAKLQN

vcParB1-linker triple mutant at 12 mg/ml. The protein construct used for SAXS contains a non-native polyhistidine tag at the N-terminus (MGMHHHHHHENLYFQG).