Isocitrate lyase 2 (Q635C, V708C, V734C)

ICL2 mutant protein

Organism: Mycobacterium tuberculosis (strain ATCC 35801 / TMC 107 / Erdman)

Monomeric molecular weight: 87.5 kDa

Oligomeric state: Monomer

Total molecular weight: 87.5 kDa

UniProt: H8F3R6 (1-766)

Sequence:

MGSSHHHHHHSSGLVPRGSHMAIAETDTEVHTPFEQDFEKDVAATQRYFDSSRFAGIIRLYTARQVVEQRGTIPVDHIVAREAAGAFYERLRELFAARKSITTFGPYSPGQAVSMKRMGIEAIYLGGWATSAKGSSTEDPGPDLASYPLSQVPDDAAVLVRALLTADRNQHYLRLQMSERQRAATPAYDFRPFIIADADTGHGGDPHVRNLIRRFVEVGVPGYHIEDQRPGTKKCGHQGGKVLVPSDEQIKRLNAARFQLDIMRVPGIIVARTDAEAANLIDSRADERDQPFLLGATKLDVPSYKSCFLAMVRRFYELGVKELNGHLLYALGDSEYAAAGGWLERQGIFGLVSDAVNAWREDGQQSIDGIFDQVESRFVAAWEDDAGLMTYGEAVADVLEFGQSEGEPIGMAPEEWRAFAARASLHAARAKAKELGADPPWDCELAKTPEGYYQIRGGIPYAIAKSLAAAPFADILWMETKTADLADARQFAEAIHAEFPDQMLAYNLSPSFNWDTTGMTDEEMRRFPEELGKMGFVFNFITYGGHQIDGVAAEEFATALRQDGMLALARLQRKMRLVESPYRTPQTLVGGPRSDAALAASSGRTATTKAMGKGSTQHQHLVQTEVPRKLLEEWLAMWSGHYQLKDKLRVQLRPCRAGSEVLELGIHGESDDKLANVIFQPIQDRRGRTILLVRDQNTFGAELRQKRLMTLIHLWLVHRFKAQAVHYCTPTDDNLYQTSKMKSHGIFTEVNQECGEIIVAEVNHPRIAELLTPDRVALRKLITKEA

ICL2 triple mutant Q635C, V708C, V734C. The protein construct used for SAXS contains an additional N-terminal polyhistidine tag (MGSSHHHHHHSSGLVPRGSH).