Peptidase S9

S9mp protein

Organism: Mycolicibacterium phlei DSM 43239 = CCUG 21000

Monomeric molecular weight: 75.9 kDa

Oligomeric state: Tetramer

Total molecular weight: 303.7 kDa

UniProt: A0A5N5URA7 (4-661)

Sequence:

MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGSTPFHDLDDYLALPRVSGLALSPDGTRLLTTIAELNDKRTEYVSAVWEIDPAGERPARRLTRGAKGESAPAFTADGDILFVAVRPTEDDDKPPAALWRLPAAGGEAYEALTLPGGVEAVRTAREADVAVVRASLLPSARDIDDDRRLRKIRKDNKISAILHTGYPIRYWDADLGPAEPHLLGLDLGDGDVRDLTAAPGGTLRESDFDVSADGRFLVTTWQRPAPGASRHSVLVRIDLDSGERTVLVEEPEADVWRPVIAPDGSGVVYIRETFSTPHAAPRMTLGYLRFGEEPVILAGDWDRWPASVTWLRDGSAVLVTADQDGRAPVFRVDVPGGSVTQLTFDDYAYTDVVTGPDGAAYALRSSYAAPPHPVRLDADGGVTELPCVALPELPGTLTELETTAADGTRVRSWLVLPAGDGKAPLLLWIHGGPLSSWNAWSWRWNPWLLAARGYAVLLPDPGLSTGYGQDFIQRGWGAWGGAPYEDLMAATDAACEHPRIDPDHTAAMGGSFGGYMANWVAGHTDRFDAIVTHASLWALDQFGATTDSSYYWLREMTREMAQANSPHNHVHNIRTPMLVIHGDKDYRVPIGEALRLWYELLTHSALPADDDGCSPHRFLYFPSENHWVLAPQHAKVWYQVVTAFLARHVLDEDVELPETLG

The S9 peptidase of M. Phlei (S9mp) was cloned and recombinantly expressed in E. coli. The recombinant S9mp protein was purified to homogeneity using Ni-IMAC, followed by size exclusion chromatography (SEC). Both SEC and dynamic light scattering confirmed tetrameric quaternary state and monodispersed. The protein construct used for SAXS contains additional N-terminal non native amino acids, including a polyhistidine tag (MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGS).