glucan 1,3-beta-glucosidase

McGlcH protein

Organism: Mucor circinelloides

Monomeric molecular weight: 46.1 kDa

Oligomeric state: Dimer

Total molecular weight: 92.2 kDa

UniProt: A0A7M3TUH9 (27-416)

Sequence:

ITTSSDSSCNLSGKSPNSNSNPDHINWRTFSGHGVNLGGWLEQESTIDTKWWSTYGANASDEWTICASLGPHRCAQVFEDRYKTFITPADIDTLAAAGVSILRIPTTYAAWVKVPGSQLYSGRQQQHLRKIADHAIHKHGMHIIIDLHSLPGGTNGLDIGERVGHWGWWFNDTALDWSLRAVDALVAFVQDSSSPQSYTIEPINEPVDNHDFSTFGTPAALSEDGAQWLARYFRAVIARVKKVNTRIPVMLQGSFKTEEYWAPFFDASENIVFDLHHYYFQFPEATSANVSTYICRDARASAGDGKFPTFVGEWAIQTGGKNELAQRGQSLRAGLSAWAQFTRGSAYWTARYFSDAAVVGEGMKEDYWSYEKFVAEGLLEGELVVKDYCHLEQKLISEEDLNSAVDHHHHHH

Signal sequence cleaved between Pro26 and Ile27 (confirmed by MALDI-TOF; predicted 24-25; UniProt numbering). The protein construct used for SAXS contains an additional polyhistidine 6x HisTag at the C-terminus for affinity purification connected to a non-native 17 amino acid linker (HLEQKLISEEDLNSAVDHHHHHH).