glucan 1,3-beta-glucosidase (long form, glycosylated)

McGlcH protein

Organism: Mucor circinelloides

Monomeric molecular weight: 46.1 kDa

Oligomeric state: Tetramer

Total molecular weight: 184.3 kDa

UniProt: A0A7M3TUH9 (27-416)

Sequence:

ITTSSDSSCNLSGKSPNSNSNPDHINWRTFSGHGVNLGGWLEQESTIDTKWWSTYGANASDEWTICASLGPHRCAQVFEDRYKTFITPADIDTLAAAGVSILRIPTTYAAWVKVPGSQLYSGRQQQHLRKIADHAIHKHGMHIIIDLHSLPGGTNGLDIGERVGHWGWWFNDTALDWSLRAVDALVAFVQDSSSPQSYTIEPINEPVDNHDFSTFGTPAALSEDGAQWLARYFRAVIARVKKVNTRIPVMLQGSFKTEEYWAPFFDASENIVFDLHHYYFQFPEATSANVSTYICRDARASAGDGKFPTFVGEWAIQTGGKNELAQRGQSLRAGLSAWAQFTRGSAYWTARYFSDAAVVGEGMKEDYWSYEKFVAEGLLEGELVVKDYCHLEQKLISEEDLNSAVDHHHHHH

Signal sequence cleaved between Pro26 and Ile27 (Uniprot numbering, confirmed by MALDI-TOF). The protein is posttranslationally N- and O- glycosylated. The protein construct used for SAXS contains an additional polyhistidine 6x HisTag at the C-terminus for affinity purification connected to a non-native 17 amino acid linker (HLEQKLISEEDLNSAVDHHHHHH).