glucan 1,3-beta-glucosidase (W90L, N179H)

AnRut protein

Organism: Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7)

Monomeric molecular weight: 41.6 kDa

Oligomeric state: Monomer

Total molecular weight: 41.6 kDa

UniProt: G3YFQ1 (20-386)

Sequence:

EFRAPLASPPNSSYIDWRTFKGNGVNLGGWLEQESTIDSLFWDKYSGGASDEWGLCEHLGSQCGPVLEHRYATLITKADIDKLASGGITVLRIPTTYAAWIDLPSSQLYSGNQTAYLKEIADYAIKTYNMHIIIDTHSLPGGVNGLTIGEATGHWYWFYNETHFNYSMQVIDQVINFIQTSGSPQSYTLEPINEPADNNTNMVVFGTPLALTDHGAAWVLKYIRAVVQRVESVNPNIPVMFQGSFKYPQYWEGDFPASTNLVFDTHHYYYEHMDSSSENLPEYILADAREKSGTGKFPVFVGEWAIQATYNNTLALRKRNVLAGLETWSSFSQGSSYWTAKFTGNTSVAGQGEQKDYWCYETFIDEGYFN

Amino acids 1-19 encode a signal peptide (UniProt numbering). The mature protein construct used for SAXS (after the removal of the signal sequence) contains an additional three non-native amino acids at the N-terminus (EFR). The protein was deglycosylated using endoglycosidase H.