Collagen alpha-3(VI) chain (D2357R, K2367R, D2431V, R2441T, R2609A, R2610A)

ColVIa3 protein

Organism: Homo sapiens

Monomeric molecular weight: 54.2 kDa

Oligomeric state: Other

Total molecular weight: 54.2 kDa

UniProt: P12111 (2347-2820)

Sequence:

ETGDYKDDDDKGGGGSGGGGSGPPGIVGQKGRPGYPGPAGPRGNRGDSIDQCALIQSIKDKCPCCYGPLECPVFPTELAFALDTSEGVNQDTFGRMRDVVLSIVNVLTIAESNCPTGARVAVVTYNNEVTTEIRFADSKRKSVLLDKIKNLQVALTSKQQSLETAMSFVARNTFKRVRNGFLMRKVAVFFSNTPTRASPQLREAVLKLSDAGITPLFLTRQEDRQLINALQINNTAVGHALVLPAGRDLTDFLENVLTCHVCLDICNIDPSCGFGSWRPSFRDAAAAGSDVDIDMAFILDSAETTTLFQFNEMKKYIAYLVRQLDMSPDPKASQHFARVAVVQHAPSESVDNASMPPVKVEFSLTDYGSKEKLVDFLSRGMTQLQGTRALGSAIEYTIENVFESAPNPRDLKIVVLMLTGEVPEQQLEEAQRVILQAKCKGYFFVVLGIGRKVNIKEVYTFASEPNDVFFKLVDKSTELNEEPLMRFGRLLPSFV

C-terminal fragment of Collagen alpha-3(VI) chain with R2609A and R2610A mutations to abolish cleavage by furin protease. Collagen VI acts as a cell-binding protein. The protein construct used for SAXS contains an additional non-native N-terminal amino acid extension (ETGDYKDDDDKGGGGSGGGGS), plus additional point mutations relative to the canonical sequence in UniProt (D2357R, K2367R, D2431V, R2441T).