Heme ligase

HDPpf protein

Organism: Plasmodium falciparum (isolate 3D7)

Monomeric molecular weight: 28.1 kDa

Oligomeric state: Unknown

Total molecular weight: 28.1 kDa

UniProt: Q8IL04

Sequence:

>STR-6His-HDPpf MASWSHPQFEKGSSHHHHHHSSGSGGGGGENLYFQGSKNRFYYNLIIKRLYTRSGGLRKPQKVTNDPESINRKVYWCFEHKPVKRTIINLIYSHNELKIFSNLLNHPTVGSSLIHELSLDGPYTAFFPSNEAMQLINIESFNKLYNDENKLSEFVLNHVTKEYWLYRDLYGSSYQPWLMYNEKREAPEKLRNLLNNDLIVKIEGEFKHCNHSIYLNGSKIIRPNMKCHNGVVHIVDKPIIF

Expression of recombinant HDPpf at 18°C resulted in minimal soluble protein. Co-expression with DnaK-DnaJ-GrpE (pKJE7) significantly improved solubility, but the protein did not bind to Ni-NTA resin. Downstream characterization confirmed that recombinant HDPpf is in complex with DnaK (DnaK-HDPpf)