Non structural Protein 15

NSP15 protein

Organism: Severe acute respiratory syndrome coronavirus 2

Monomeric molecular weight: 233.3 kDa

Oligomeric state: Monomer

Total molecular weight: 250.7 kDa

UniProt: P0DTD1 (6465-6798)

Sequence:

MTIFAPQHKADFGIIDGHFNNFANNAQILAFNDFHKIIQQQINNAANTFAATGLCIGDKIVFHGAIHRNEILKFKRAFFKTFGQTNQQMQTTLAMAKITIHDMFKGIAFQFITFDKFIHGQFQKINFHLATRFKFLQITALGKIGFRQLLNNAIDFFVIVELGFNCFTNQGNTIQARLFRTNARLQTFNATFGNQNTITCITKQVNLTIHTAIKKNGQRCTNGFGRFFCNIGHATNTNGANMCRCITFIIPDNGIGCNINTQIIQNFHFRNRFDITFCPQFKRNINRQCGFILKQFNINTIHFGIHGIVNNANRNFTLLTIKMAFVHNIKGNIFQAGSMRP

The sample comprises a hexameric assembly of NSP15 protein (monomer MW ≈ 38.88 kDa) with an estimated hexameric molecular weight of ~233.30 kDa. Each protein hexamer is complexed with six copies of a 9-nucleotide single-stranded RNA (sequence: AAACGACAU), contributing an additional ~17.45 kDa, yielding a total molecular weight of approximately 250.74 kDa. The RNA is presumed to bind symmetrically across the RNA-binding sites of the protein hexamer, maintaining a compact, globular assembly in solution.