Upstream of N-Ras C-terminal cold shock domains mediate poly(A) specificity in a novel RNA recognition mode and bind poly(A) binding protein.

Hollmann NM, Jagtap PKA, Linse JB, Ullmann P, Payr M, Murciano B, Simon B, Hub JS, Hennig J
Nucleic Acids Res (2023 Jan 23)

PMID: 36688322 doi: 10.1093/nar/gkac1277

Submitted to SASBDB: 2020 Feb 27 Published in SASBDB: 2023 Jan 26

SASDHH7 – Upstream of N-ras, isoform A, CSD 7, 8 and 9 from Drosophila melanogaster Sample: Upstream of N-ras, isoform A monomer, 26 kDa Drosophila melanogaster protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 19 RgGuinier 2.3 nm Dmax 9.0 nm VolumePorod 38 nm3

SASDHL7 – Upstream of N-ras, isoform A, CSD 4 to 9 from Drosophila melanogaster Sample: Upstream of N-ras, isoform A monomer, 63 kDa protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2019 Sep 30 RgGuinier 4.9 nm Dmax 25.0 nm VolumePorod 136 nm3

SASDHM7 – Upstream of N-ras, isoform A, CSD 1 to 6 from Drosophila melanogaster Sample: Upstream of N-ras, isoform A monomer, 57 kDa Drosophila melanogaster protein Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Sep 30 RgGuinier 4.4 nm Dmax 20.0 nm VolumePorod 160 nm3

SASDNN8 – Upstream of N-ras, isoform A, CSD 7, 8 and 9 from Drosophila melanogaster in complex with a poly(A)-15mer RNA Sample: Upstream of N-ras, isoform A monomer, 26 kDa Drosophila melanogaster protein PolyA-15mer monomer, 5 kDa RNA Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 8 RgGuinier 2.2 nm Dmax 7.5 nm VolumePorod 37 nm3