Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism

Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R
(2020 Apr 24)

doi: 10.1101/2020.04.22.056440

Submitted to SASBDB: 2020 Apr 7 Published in SASBDB: 2020 Jun 9

SASDHR9 – GntR-type transcriptional regulator NanR from Escherichia coli Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20 RgGuinier 3.2 nm Dmax 10.5 nm VolumePorod 110 nm3

SASDHS9 – GntR-type transcriptional regulator NanR from Escherichia coli in the presence of N-acetylneuraminic acid Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein Buffer: 20 mM Tris, 150 mM NaCl, 20 mM Neu5Ac and 0.1 % (w/v) sodium azide, pH: 8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20 RgGuinier 3.1 nm Dmax 10.1 nm VolumePorod 105 nm3

SASDHT9 – Dimeric NanR-DNA hetero-complex from Escherichia coli Sample: HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein (GGTATA)2 repeat DNA monomer, 11 kDa DNA Buffer: 20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20 RgGuinier 3.3 nm Dmax 9.8 nm VolumePorod 108 nm3