Towards improved understanding of intersubunit interactions in modular polyketide biosynthesis: docking in the enacyloxin IIa polyketide synthase.

Risser F, Collin S, Dos Santos-Morais R, Gruez A, Chagot B, Weissman KJ
J Struct Biol :107581 (2020 Jul 24)
PMID: 32717326
doi: 10.1016/j.jsb.2020.107581
Submitted to SASBDB: 2020 Apr 17
Published in SASBDB:

SASDJE2 – Beta-ketoacyl synthase (Bamb_5925) C-terminal docking domain

Beta-ketoacyl synthase Bamb_5925 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5925 monomer, 4 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 1.6 nm
Dmax 6.2 nm
VolumePorod 3 nm3

SASDJF2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain

Beta-ketoacyl synthase Bamb_5924 experimental SAS data
DAMFILT model
Sample: Beta-ketoacyl synthase Bamb_5924 dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 6
RgGuinier 1.9 nm
Dmax 7.5 nm
VolumePorod 22 nm3

SASDJG2 – Beta-ketoacyl synthase (Bamb_5920) C-terminal docking domain

Beta-ketoacyl synthase Bamb_5920 experimental SAS data
DAMMIN model
Sample: Beta-ketoacyl synthase Bamb_5920 monomer, 2 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 1.2 nm
Dmax 4.9 nm
VolumePorod 3 nm3

SASDJH2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain mutant K32A

Beta-ketoacyl synthase Bamb_5924, K32A mutant experimental SAS data
Beta-ketoacyl synthase Bamb_5924, K32A mutant Kratky plot
Sample: Beta-ketoacyl synthase Bamb_5924, K32A mutant dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 1.8 nm
Dmax 8.0 nm
VolumePorod 22 nm3

SASDJJ2 – Beta-ketoacyl synthase (Bamb_5924) N-terminal docking domain mutant R17A

Beta-ketoacyl synthase Bamb_5924, R17A mutant experimental SAS data
Beta-ketoacyl synthase Bamb_5924, R17A mutant Kratky plot
Sample: Beta-ketoacyl synthase Bamb_5924, R17A mutant dimer, 10 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 1.8 nm
Dmax 8.2 nm
VolumePorod 20 nm3

SASDJK2 – Artificial fusion protein of the beta-ketoacyl synthase Bamb_5920 C-terminal docking domain and the beta-ketoacyl synthase Bamb_5919 N-terminal docking domain

Beta-ketoacyl synthase Bamb_5920 CDD/Bamb_5919 NDD artificial protein fusion experimental SAS data
DAMFILT model
Sample: Beta-ketoacyl synthase Bamb_5920 CDD/Bamb_5919 NDD artificial protein fusion dimer, 13 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 1.8 nm
Dmax 6.0 nm
VolumePorod 25 nm3

SASDJL2 – Artificial fusion protein of the beta-ketoacyl synthase Bamb_5925 C-terminal docking domain and the beta-ketoacyl synthase Bamb_5924 N-terminal docking domain

Beta-ketoacyl synthase Bamb_5925 CDD/Bamb_5924  NDD artificial protein fusion experimental SAS data
PDB model
Sample: Beta-ketoacyl synthase Bamb_5925 CDD/Bamb_5924 NDD artificial protein fusion dimer, 18 kDa Burkholderia ambifaria protein
Buffer: 20 mM Tris-HCl, 200 mM NaCl, 5% glycerol, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jan 23
RgGuinier 2.1 nm
Dmax 9.6 nm
VolumePorod 47 nm3