Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins.

Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
J Mol Biol (2020 Aug 26)

PMID: 32860773 doi: 10.1016/j.jmb.2020.08.020

Submitted to SASBDB: 2020 May 29 Published in SASBDB: 2020 Sep 2

SASDJS3 – Adenylate cyclase toxin CyaA RTX block II-V Sample: Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 4.0 nm Dmax 13.3 nm VolumePorod 94 nm3

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V Sample: Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 3.4 nm Dmax 12.1 nm VolumePorod 85 nm3

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V Sample: Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 2.7 nm Dmax 8.6 nm VolumePorod 45 nm3