Characterization of the Pilotin-Secretin Complex from the Salmonella enterica Type III Secretion System Using Hybrid Structural Methods.

Majewski DD, Okon M, Heinkel F, Robb CS, Vuckovic M, McIntosh LP, Strynadka NCJ
Structure (2020 Aug 27)
PMID: 32877645
doi: 10.1016/j.str.2020.08.006
Submitted to SASBDB: 2020 Jun 16
Published in SASBDB:

SASDJR4 – Type 3 secretion system pilotin, InvH27-147

Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 28 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
RgGuinier 3.1 nm
Dmax 12.6 nm
VolumePorod 53 nm3

SASDJS4 – Type 3 secretion system pilotin, InvH70-147

Type 3 secretion system pilotin experimental SAS data
DAMMIN model
Sample: Type 3 secretion system pilotin dimer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, University of British Columbia on 2017 Mar 23
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 29 nm3