Structural Insights into the Mechanism of a Nanobody That Stabilizes PAI-1 and Modulates Its Activity

Sillen M, Weeks S, Strelkov S, Declerck P
International Journal of Molecular Sciences 21(16):5859 (2020 Aug 15)

doi: 10.3390/ijms21165859 		Submitted to SASBDB: 2020 Jul 23
Published in SASBDB:

SASDJW5 – Plasminogen activator inhibitor-1 (PAI-1) W175F bound to nanobody Nb93

Plasminogen activator inhibitor 1VHH-s-a93 (Ig module Nb93) experimental SAS data
OTHER model
Sample: Plasminogen activator inhibitor 1 monomer, 43 kDa Homo sapiens protein
VHH-s-a93 (Ig module Nb93) monomer, 13 kDa Vicugna pacos protein
Buffer: 30 mM BIS-TRIS pH 5.5, 300 mM sodium chloride, 5% v/v glycerol, pH: 5.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 5
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 80 nm3