Solution structure(s) of trinucleosomes from contrast variation SAXS

Mauney A, Muthurajan U, Luger K, Pollack L
Nucleic Acids Research (2021 May 01)

doi: 10.1093/nar/gkab290 		Submitted to SASBDB: 2020 Jul 31
Published in SASBDB:

SASDJ96 – Trinucleosomes from Xenopus laevis (African clawed frog)

Histone H3Histone H4Histone H2A type 1Histone H2BNon-linker Ended Trinucleosome DNA experimental SAS data
Histone H3 Histone H4 Histone H2A type 1 Histone H2B Non-linker Ended Trinucleosome DNA Kratky plot
Sample: Histone H3 monomer, 15 kDa Xenopus laevis protein
Histone H4 monomer, 11 kDa Xenopus laevis protein
Histone H2A type 1 monomer, 14 kDa Xenopus laevis protein
Histone H2B monomer, 14 kDa Xenopus laevis protein
Non-linker Ended Trinucleosome DNA monomer, 172 kDa DNA
Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 1 mM DTT 50% w/v sucrose, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Mar 11
RgGuinier 12.9 nm
Dmax 41.8 nm