Low-resolution structures of modular nanotransporters shed light on their functional activity

Khramtsov Y, Vlasova A, Vlasov A, Rosenkranz A, Ulasov A, Ryzhykau Y, Kuklin A, Orekhov A, Eydlin I, Georgiev G, Gordeliy V, Sobolev A
Acta Crystallographica Section D Structural Biology 76(12):1270-1279 (2020 Nov 24)

doi: 10.1107/S2059798320013765 		Submitted to SASBDB: 2020 Sep 14
Published in SASBDB:

SASDJY7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH)

Modular nanotransporter with a melanocyte stimulating hormone ligand module experimental SAS data
A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH) Rg histogram
Sample: Modular nanotransporter with a melanocyte stimulating hormone ligand module monomer, 70 kDa synthetic construct protein
Buffer: 150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24
RgGuinier 3.7 nm
Dmax 13.3 nm
VolumePorod 127 nm3

SASDJZ7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to an epidermal growth factor ligand module (MNT-EGF)

Modular nanotransporter with an epidermal growth factor ligand module experimental SAS data
GASBOR model
Sample: Modular nanotransporter with an epidermal growth factor ligand module monomer, 76 kDa synthetic construct protein
Buffer: 150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 161 nm3