Low-resolution structures of modular nanotransporters shed light on their functional activity

Yury Ryzhykau Submitted to SASBDB: 2020 Sep 14
Published in SASBDB:

SASDJY7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH)

Modular nanotransporter with a melanocyte stimulating hormone ligand module experimental SAS data
A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to a melanocyte stimulating hormone ligand module (MNT-MSH) Rg histogram
Sample: Modular nanotransporter with a melanocyte stimulating hormone ligand module monomer, 70 kDa synthetic construct protein
Buffer: 150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24
RgGuinier 3.7 nm
Dmax 13.3 nm
VolumePorod 127 nm3

SASDJZ7 – A modular diphtheria toxin/flavohemoprotein nanotransporter (MNT) fused to an epidermal growth factor ligand module (MNT-EGF)

Modular nanotransporter with an epidermal growth factor ligand module experimental SAS data
GASBOR model
Sample: Modular nanotransporter with an epidermal growth factor ligand module monomer, 76 kDa synthetic construct protein
Buffer: 150 mM NaCl, 10 mM phosphate buffer saline, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Nov 24
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 161 nm3