Structural insights into the substrate-binding proteins Mce1A and Mce4A from Mycobacterium tuberculosis

Asthana P, Singh D, Pedersen J, Hynönen M, Sulu R, Murthy A, Laitaoja M, Jänis J, Riley L, Venkatesan R
IUCrJ 8(5) (2021 Jul 28)

doi: 10.1107/S2052252521006199 		Submitted to SASBDB: 2020 Oct 9
Published in SASBDB:

SASDJU9 – Mammalian cell entry protein 1A (Mce1A36-148)

Mce-family protein Mce1A monomer experimental SAS data
DAMMIN model
Sample: Mce-family protein Mce1A monomer monomer, 17 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris, 350 mM NaCl, 10% Glycerol, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 10
RgGuinier 2.1 nm
Dmax 8.7 nm
VolumePorod 29 nm3

SASDK32 – Mammalian cell entry protein 1A (Mce1A38-454)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 48 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 112 kDa
Buffer: 50 mM Tris, 500 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 13
RgGuinier 5.3 nm
Dmax 21.6 nm
VolumePorod 362 nm3

SASDK22 – Mammalian cell entry protein 1A (Mce1A126-454)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 39 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 123 kDa
Buffer: 50 mM Tris, 350 mM NaCl, 10% Glycerol, 5mM DDM, 1 mM β-ME, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Nov 28
RgGuinier 5.5 nm
Dmax 22.0 nm
VolumePorod 216 nm3

SASDJZ9 – Mammalian cell entry protein 1A (Mce1A38-325)

Mce-family protein Mce1An-Dodecyl-β-D-Maltopyranoside experimental SAS data
CUSTOM IN-HOUSE model
Sample: Mce-family protein Mce1A monomer, 36 kDa Mycobacterium tuberculosis protein
N-Dodecyl-β-D-Maltopyranoside 0, 82 kDa
Buffer: 50 mM Tris, 500 mM NaCl, 10% Glycerol, 5 mM DDM, 1 mM β-ME, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 2
RgGuinier 4.6 nm
Dmax 17.5 nm
VolumePorod 151 nm3

SASDJV9 – Mammalian cell entry protein 4A (Mce4A39-140)

Mce-family protein Mce4A experimental SAS data
DAMMIN model
Sample: Mce-family protein Mce4A monomer, 15 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM MOPS, 350mM NaCl, 10% Glycerol, pH: 7
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 10
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 22 nm3

SASDJW9 – Mammalian cell entry protein 4A (Mce4A36-400)

Mce-family protein Mce4An-Dodecyl-β-D-Maltopyranoside experimental SAS data
OTHER model
Sample: Mce-family protein Mce4A monomer, 44 kDa Mycobacterium tuberculosis (strain … protein
N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 13
RgGuinier 5.7 nm
Dmax 21.5 nm
VolumePorod 446 nm3

SASDJX9 – Mammalian cell entry protein 4A (Mce4A121-400)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data
CUSTOM IN-HOUSE model
Sample: N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 35 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Nov 28
RgGuinier 5.0 nm
Dmax 19.1 nm
VolumePorod 278 nm3

SASDJY9 – Mammalian cell entry protein 4A (Mce4A39-320)

n-Dodecyl-β-D-MaltopyranosideMce-family protein Mce4A experimental SAS data
CUSTOM IN-HOUSE model
Sample: N-Dodecyl-β-D-Maltopyranoside 0, 102 kDa
Mce-family protein Mce4A monomer, 34 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50mM Tris, 500mM NaCl, 10% Glycerol, 5mM DDM, 1mM Beta-ME, pH: 8.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 May 2
RgGuinier 4.8 nm
Dmax 18.2 nm
VolumePorod 189 nm3