Structural Analysis of the Menangle Virus P Protein Reveals a Soft Boundary between Ordered and Disordered Regions

Webby M, Herr N, Bulloch E, Schmitz M, Keown J, Goldstone D, Kingston R
Viruses 13(9):1737 (2021 Aug 31)

doi: 10.3390/v13091737 		Submitted to SASBDB: 2021 Jul 5
Published in SASBDB:

SASDLG9 – Menangle virus (MenV) phosphoprotein, amino acids 209-388 (coiled-coil/flexible linker/binding domain and C352S mutation)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein tetramer, 79 kDa Menangle virus protein
Buffer: 12.5 mM MOPS/KOH pH 7.0, 250 mM NaCl, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Nov 16
RgGuinier 6.3 nm
Dmax 23.4 nm
VolumePorod 524 nm3

SASDLH9 – Menangle virus (MenV) phosphoprotein, amino acids 267-388 (flexible linker/binding domain and C352S mutation)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein monomer, 13 kDa Menangle virus protein
Buffer: 12.5 mM MOPS/KOH pH 7.0, 150 mM NaCl, pH: 7
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Aug 17
RgGuinier 3.1 nm
Dmax 12.2 nm
VolumePorod 20 nm3

SASDLJ9 – Menangle virus (MenV) phosphoprotein C-terminal fragment, amino acids 267-328 (flexible linker)

Phosphoprotein experimental SAS data
Phosphoprotein Kratky plot
Sample: Phosphoprotein monomer, 6 kDa Menangle virus protein
Buffer: 12.5 mM Tris/HCl pH 8.5, 150 mM NaCl, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 15
RgGuinier 2.5 nm
Dmax 10.3 nm
VolumePorod 12 nm3