Designed folding pathway of modular coiled-coil-based proteins.

Aupič J, Strmšek Ž, Lapenta F, Pahovnik D, Pisanski T, Drobnak I, Ljubetič A, Jerala R
Nat Commun 12(1):940 (2021 Feb 11)
PMID: 33574262
doi: 10.1038/s41467-021-21185-5 		Submitted to SASBDB: 2020 Nov 2
Published in SASBDB:

SASDKQ2 – TET12SN(2CC)

TET12(1.10)SN-f5(2CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(2CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Dec 13
RgGuinier 3.4 nm
Dmax 9.6 nm
VolumePorod 158 nm3

SASDKR2 – TET12SN(22CC)

TET12(1.10)SN-f5(22CC) experimental SAS data
DAMMIF model
Sample: TET12(1.10)SN-f5(22CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 5
RgGuinier 3.4 nm
Dmax 10.4 nm
VolumePorod 165 nm3

SASDKS2 – TET12SN(222CC)

TET12(1.10)SN-f5(222CC) experimental SAS data
MODELLER model
Sample: TET12(1.10)SN-f5(222CC) monomer, 55 kDa synthetic construct protein
Buffer: 20 mM Tris 150 mM NaCl 10% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 15
RgGuinier 3.4 nm
Dmax 10.6 nm
VolumePorod 170 nm3