Activity of lymphostatin, a lymphocyte inhibitory virulence factor of pathogenic Escherichia coli, is dependent on a cysteine protease motif.

Bease AG, Blackburn EA, Chintoan-Uta C, Webb S, Cassady-Cain RL, Stevens MP
J Mol Biol :167200 (2021 Aug 13)

PMID: 34400181 doi: 10.1016/j.jmb.2021.167200

Submitted to SASBDB: 2021 Mar 4 Published in SASBDB: 2021 Aug 26

SASDKL9 – Lymphostatin pH7.5 Sample: Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein Buffer: 50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29 RgGuinier 5.9 nm Dmax 19.3 nm VolumePorod 559 nm3

SASDKM9 – Lymphostatin pH5.5 Sample: Efa1/LifA protein monomer, 367 kDa Escherichia coli O127:H6 … protein Buffer: 50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29 RgGuinier 5.9 nm Dmax 19.7 nm VolumePorod 565 nm3

SASDKN9 – Lymphostatin C1480A mutant pH 7.5 Sample: Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein Buffer: 50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29 RgGuinier 5.9 nm Dmax 19.1 nm VolumePorod 557 nm3

SASDKP9 – Lymphostatin C1480A mutant pH5.5 Sample: Efa1/LifA protein (C1480A mutant) monomer, 367 kDa Escherichia coli O127:H6 … protein Buffer: 50 mM Bis-Tris, 100 mM NaCl, 3 % (v/v) glycerol, pH: 5.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29 RgGuinier 5.8 nm Dmax 19.3 nm VolumePorod 560 nm3