Structural Insights into the Unique Modes of Relaxin-Binding and Tethered-Agonist Mediated Activation of RXFP1 and RXFP2.

Sethi A, Bruell S, Ryan T, Yan F, Tanipour MH, Mok YF, Draper-Joyce C, Khandokar Y, Metcalfe RD, Griffin MDW, Scott DJ, Hossain MA, Petrie EJ, Bathgate RAD, Gooley PR
J Mol Biol 433(21):167217 (2021 Oct 15)

PMID: 34454945 doi: 10.1016/j.jmb.2021.167217

Submitted to SASBDB: 2021 Jun 28 Published in SASBDB: 2021 Nov 1

SASDLG7 – LDLa linker region of human Relaxin receptor 1 (RXFP1, 23-94) Sample: Relaxin receptor 1 monomer, 8 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6 RgGuinier 2.1 nm Dmax 10.0 nm VolumePorod 19 nm3

SASDLH7 – LDLa linker region of human Relaxin receptor 2 (RXFP2, 38-105) Sample: Relaxin receptor 2 monomer, 7 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.1%NaN3, pH: 7.4 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 6 RgGuinier 1.7 nm Dmax 7.1 nm VolumePorod 12 nm3