Structure and dynamics of the von Willebrand Factor C6 domain

Emma-Ruoqi Xu Submitted to SASBDB: 2021 Jul 25
Published in SASBDB:

SASDLR9 – von Willebrand factor C6 domain G2705K mutant

von Willebrand factor C6 domain G2705K mutant experimental SAS data
von Willebrand factor C6 domain G2705K mutant Kratky plot
Sample: Von Willebrand factor C6 domain G2705K mutant monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 2
RgGuinier 1.8 nm
Dmax 6.7 nm
VolumePorod 11 nm3

SASDLS9 – von Willebrand factor C6-CK wildtype

von Willebrand factor C6-CK wildtype experimental SAS data
von Willebrand factor C6-CK wildtype Kratky plot
Sample: Von Willebrand factor C6-CK wildtype dimer, 41 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 18
RgGuinier 3.3 nm
Dmax 15.0 nm
VolumePorod 90 nm3

SASDLT9 – von Willebrand factor C6-CK G2705R

von Willebrand factor C6-CK G2705R experimental SAS data
von Willebrand factor C6-CK G2705R Kratky plot
Sample: Von Willebrand factor C6-CK G2705R dimer, 42 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 18
RgGuinier 3.5 nm
Dmax 16.5 nm
VolumePorod 95 nm3

SASDLU9 – von Willebrand factor C6-CK G2705K

von Willebrand factor C6-CK G2705K experimental SAS data
von Willebrand factor C6-CK G2705K Kratky plot
Sample: Von Willebrand factor C6-CK G2705K dimer, 41 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Aug 18
RgGuinier 3.5 nm
Dmax 16.0 nm
VolumePorod 106 nm3

SASDLV9 – von Willebrand factor D4N-CK wildtype

von Willebrand factor D4N-CK wildtype experimental SAS data
von Willebrand factor D4N-CK wildtype Kratky plot
Sample: Von Willebrand factor D4N-CK wildtype dimer, 211 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23
RgGuinier 7.8 nm
Dmax 34.8 nm
VolumePorod 637 nm3

SASDLW9 – von Willebrand factor D4N-CK G2705R

von Willebrand factor D4N-CK G2705R experimental SAS data
von Willebrand factor D4N-CK G2705R Kratky plot
Sample: Von Willebrand factor D4N-CK G2705R dimer, 211 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23
RgGuinier 11.4 nm
Dmax 68.5 nm
VolumePorod 1800 nm3

SASDLX9 – von Willebrand factor D4N-CK G2705K

von Willebrand factor D4N-CK G2705K experimental SAS data
von Willebrand factor D4N-CK G2705K Kratky plot
Sample: Von Willebrand factor D4N-CK G2705K dimer, 211 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23
RgGuinier 9.8 nm
Dmax 52.0 nm
VolumePorod 917 nm3