Reconstruction of Quaternary Structure from X-ray Scattering by Equilibrium Mixtures of Biological Macromolecules

Petoukhov M, Billas I, Takacs M, Graewert M, Moras D, Svergun D
Biochemistry 52(39):6844-6855 (2013 Sep 19)

doi: 10.1021/bi400731u

Submitted to SASBDB: 2021 Aug 5 Published in SASBDB: 2021 Sep 10

SASDMZ4 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 2.9 mg/ml Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3 RgGuinier 4.7 nm Dmax 14.6 nm

SASDM25 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.9 mg/ml Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3 RgGuinier 4.3 nm Dmax 13.2 nm

SASDM35 – Mixture of estrogen-related receptor gamma:Inverse repeat IR3 DNA - 6:3 and 2:1 complexes - at 1.0 mg/ml Sample: Estrogen-related receptor gamma hexamer, 230 kDa Homo sapiens protein Inverse repeat IR3 DNA trimer, 46 kDa DNA Buffer: 20 mM Tris-HCl, 100 mM NaCl, 100 mM KCl, 5 mM MgCl2, 1% (v/v) glycerol, and 1 mM CHAPS, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Mar 3 RgGuinier 4.1 nm Dmax 12.8 nm