Dystrophin's central domain forms a complex filament that becomes disorganized by in-frame deletions.

Delalande O, Molza AE, Dos Santos Morais R, Chéron A, Pollet É, Raguenes-Nicol C, Tascon C, Giudice E, Guilbaud M, Nicolas A, Bondon A, Leturcq F, Férey N, Baaden M, Perez J, Roblin P, Piétri-Rouxel F, Hubert JF, Czjzek M, Le Rumeur E
J Biol Chem 293(18):6637-6646 (2018 May 4)

PMID: 29535188 doi: 10.1074/jbc.M117.809798

Submitted to SASBDB: 2016 Jan 20 Published in SASBDB: 2018 Apr 21

SASDB43 – Human dystrophin central domain repeats 1 to 2 Sample: Dystrophin central domain repeats 1 to 2 monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Oct 7 RgGuinier 3.0 nm Dmax 10.6 nm VolumePorod 42 nm3

SASDB53 – Human dystrophin central domain repeats 1 to 3 Sample: Dystrophin central domain repeats 1 to 3 monomer, 38 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Sep 9 RgGuinier 4.2 nm Dmax 17.0 nm VolumePorod 68 nm3

SASDB63 – Human dystrophin central domain repeats 11 to 15 Sample: Dystrophin central domain repeats 11 to 15. monomer, 60 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2011 Apr 11 RgGuinier 5.8 nm Dmax 23.0 nm VolumePorod 87 nm3

SASDB73 – Human dystrophin central domain repeats 4 to 9 Sample: Dystrophin central domain repeats 4 to 9 monomer, 76 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Sep 19 RgGuinier 7.7 nm Dmax 30.5 nm VolumePorod 123 nm3

SASDB83 – Human dystrophin central domain repeats 16 to 17 Sample: Dystrophin central domain repeats 16 to 17. monomer, 28 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2012 Sep 19 RgGuinier 3.1 nm Dmax 13.0 nm VolumePorod 47 nm3

SASDB93 – Human dystrophin central domain repeats 16 to 19 Sample: Dystrophin central domain repeats 16 to 19. monomer, 50 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol 5% acetonitrile, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2013 Oct 4 RgGuinier 4.6 nm Dmax 20.0 nm VolumePorod 70 nm3

SASDBA3 – Human dystrophin central domain repeats 20 to 24 Sample: Dystrophin central domain repeats 20 to 24. monomer, 67 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Jul 10 RgGuinier 5.8 nm Dmax 22.5 nm VolumePorod 107 nm3

SASDBB3 – Human dystrophin central domain single repeat 23 Sample: Dystrophin central domain single repeat 23 monomer, 17 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Oct 7 RgGuinier 2.2 nm Dmax 7.4 nm VolumePorod 20 nm3

SASDBV3 – Dystrophin central domain repeats 16 to 21 (Δ2146-2305; Becker muscular dystrophy variant) Sample: Dystrophin central domain repeats 16 to 21 (Δ2146-2305; Becker muscular dystrophy variant, deletion of exons 45-47) monomer, 64 kDa Homo sapiens protein Buffer: 20 mM Tris 150 mM NaCl 1 mM EDTA 2% glycerol 5% acetonitrile, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2014 Feb 5 RgGuinier 6.0 nm Dmax 21.0 nm VolumePorod 184 nm3