Reconstitution of the DTX3L-PARP9 complex reveals determinants for high affinity heterodimerization and multimeric assembly.

Ashok Y, Vela-Rodríguez C, Yang CS, Alanen HI, Liu F, Paschal BM, Lehtiö L
Biochem J (2022 Jan 17)
PMID: 35037691
doi: 10.1042/BCJ20210722 		Submitted to SASBDB: 2021 Oct 8
Published in SASBDB:

SASDMQ6 – The N-terminus of E3 ubiquitin-protein ligase DTX3L (M1-Q200)

E3 ubiquitin-protein ligase DTX3L experimental SAS data
E3 ubiquitin-protein ligase DTX3L Kratky plot
Sample: E3 ubiquitin-protein ligase DTX3L pentamer, 114 kDa Homo sapiens protein
Buffer: 30 mM HEPES, 350 mM NaCl, 10% glycerol, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 6
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 200 nm3