Centriolar cap proteins CP110 and CPAP control slow elongation of microtubule plus ends.

Iyer SS, Chen F, Ogunmolu FE, Moradi S, Volkov VA, van Grinsven EJ, van Hoorn C, Wu J, Andrea N, Hua S, Jiang K, Vakonakis I, Potočnjak M, Herzog F, Gigant B, Gudimchuk N, Stecker KE, Dogterom M, Steinmetz MO, Akhmanova A
J Cell Biol 224(3) (2025 Mar 3)
PMID: 39847124
doi: 10.1083/jcb.202406061 		Submitted to SASBDB: 2021 Dec 21
Published in SASBDB:

SASDNA3 – Association of the CPAP N-termini coiled-coil domain with the CP110 C-termini coiled-coil region

centrosomal-P4.1-associated-proteinCentriolar coiled-coil protein of 110 kDa experimental SAS data
DAMMIF model
Sample: Centrosomal-P4.1-associated-protein monomer, 12 kDa Homo sapiens protein
Centriolar coiled-coil protein of 110 kDa dimer, 20 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 23
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 35 nm3

SASDNB3 – Homodimerisation of the CP110 C-terminus coiled-coil domain

Centriolar coiled-coil protein of 110 kDa experimental SAS data
DAMMIF model
Sample: Centriolar coiled-coil protein of 110 kDa dimer, 20 kDa Homo sapiens protein
Buffer: 50 mM HEPES, pH 7.5, 100 mM NaCl, 1 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 23
RgGuinier 3.5 nm
Dmax 12.5 nm
VolumePorod 33 nm3