Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic

Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R
Biophysical Journal 94(1):173-181 (2008 Jan)

doi: 10.1529/biophysj.107.114314

Submitted to SASBDB: 2021 Dec 21 Published in SASBDB: 2022 Jan 4

SASDNN2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb2) at concentration c = 21 mg/ml Sample: Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 3.0 nm

SASDNP2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb5) Sample: Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 3.3 nm Dmax 13.0 nm

SASDNQ2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2) at concentration c = 25 mg/ml Sample: Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 2.4 nm

SASDNR2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2) Sample: Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 2.8 nm Dmax 13.0 nm

SASDNS2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 2.4 nm Dmax 13.0 nm

SASDNT2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 31.25 mg/ml Sample: Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein Buffer: Ringer's lactate solution, pH: 6.5 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19 RgGuinier 2.2 nm