Structural basis for topological regulation of Tn3 resolvase.

MontaƱo SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
Nucleic Acids Res (2022 Sep 14)
PMID: 36100255
doi: 10.1093/nar/gkac733
Submitted to SASBDB: 2022 Mar 18
Published in SASBDB:

SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence

Transposon Tn3 resolvaseTn3 res, resolvase binding site II experimental SAS data
Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein
Tn3 res, resolvase binding site II monomer, 32 kDa DNA
Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6
RgGuinier 5.0 nm
Dmax 14.9 nm
VolumePorod 217 nm3