Structural basis for topological regulation of Tn3 resolvase.

Montaño SP, Rowland SJ, Fuller JR, Burke ME, MacDonald AI, Boocock MR, Stark WM, Rice PA
Nucleic Acids Res (2022 Sep 14)

PMID: 36100255 doi: 10.1093/nar/gkac733

Submitted to SASBDB: 2022 Mar 18 Published in SASBDB: 2022 Sep 22

SASDNH7 – Tn3 resolvase dimer bound to site II DNA sequence Sample: Transposon Tn3 resolvase dimer, 43 kDa Escherichia coli protein Tn3 res, resolvase binding site II monomer, 32 kDa DNA Buffer: 25 mM Tris, 150 mM NaCl, 10 mM MgCl2, 1% glycerol, pH: 7.5 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2014 Nov 6 RgGuinier 5.0 nm Dmax 14.9 nm VolumePorod 217 nm3