Coiled-coil structure of meiosis protein TEX12 and conformational regulation by its C-terminal tip

Dunce J, Salmon L, Davies O
Communications Biology 5(1) (2022 Sep 07)

doi: 10.1038/s42003-022-03886-9

Submitted to SASBDB: 2022 Feb 8 Published in SASBDB: 2022 Sep 7

SASDNN5 – Meiosis protein TEX12 (Testis-expressed protein 12) – wild-type Sample: Testis-expressed protein 12 dimer, 18 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10 RgGuinier 2.1 nm Dmax 6.6 nm VolumePorod 27 nm3

SASDNP5 – Meiosis protein TEX12 (Testis-expressed protein 12) – delta Ctip Sample: Testis-expressed protein 12 tetramer, 32 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 10 RgGuinier 3.0 nm Dmax 11.0 nm VolumePorod 44 nm3

SASDNQ5 – Meiosis protein TEX12 (Testis-expressed protein 12) – L110E, F114E, I117E, L121E mutant Sample: Testis-expressed protein 12 (L110E, F114E, I117E, L121E) tetramer, 36 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 15 RgGuinier 3.3 nm Dmax 12.9 nm VolumePorod 59 nm3

SASDNR5 – Meiosis protein TEX12 (Testis-expressed protein 12) – F102A, F109E, V116A mutant Sample: Testis-expressed protein 12 (F102A, F109E, V116A) tetramer, 36 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Nov 27 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 48 nm3

SASDNS5 – Meiosis protein TEX12 (Testis-expressed protein 12) – F109E mutant Sample: Testis-expressed protein 12 (F109E) dimer, 18 kDa Homo sapiens protein Buffer: 20 mM Tris, 150 mM KCl, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 13 RgGuinier 2.3 nm Dmax 7.5 nm VolumePorod 30 nm3