Herpes simplex virus 1 protein pUL21 alters ceramide metabolism by activating the inter-organelle transport protein CERT

Benedyk T, Connor V, Caroe E, Shamin M, Svergun D, Deane J, Jeffries C, Crump C, Graham S
Journal of Biological Chemistry :102589 (2022 Oct)

doi: 10.1016/j.jbc.2022.102589

Submitted to SASBDB: 2022 Mar 22 Published in SASBDB: 2022 Sep 30

SASDNB7 – C-terminal domain of pUL21 with N-terminal His-tag Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25 RgGuinier 2.2 nm Dmax 8.5 nm VolumePorod 49 nm3

SASDNC7 – miniCERTL with N-terminal His-tag Sample: Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25 RgGuinier 2.7 nm Dmax 9.1 nm VolumePorod 71 nm3

SASDND7 – Complex of H6-pUL21C and H6-miniCERTL Sample: Tegument protein UL21 (C-terminal domain) monomer, 29 kDa Human herpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25 RgGuinier 3.3 nm Dmax 13.6 nm VolumePorod 84 nm3

SASDNE7 – Complex of H6-miniCERTL and full-length pUL21-H6 Sample: Tegument protein UL21 monomer, 59 kDa Human alphaherpesvirus 1 … protein Ceramide transfer protein (recombinant CERTL: amino acids 20-130 and 351-624) monomer, 45 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.5, 500 mM NaCl, 3% (v/v) glycerol, 1 mM DTT, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Sep 25 RgGuinier 4.1 nm Dmax 15.8 nm VolumePorod 158 nm3