The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.

Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM
J Biol Chem 291(36):18643-62 (2016 Sep 2)

PMID: 27413182 doi: 10.1074/jbc.M116.732909

Submitted to SASBDB: 2016 Jul 14 Published in SASBDB: 2016 Jul 14

SASDBA4 – Plakin domain fragment of Human plectin (central region spectrin repeats: SR3-SR4-SR5-SR6 and SH3) Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR4-SR5-SR6 and SH3 monomer, 53 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 5.1 nm Dmax 21.0 nm VolumePorod 91 nm3

SASDBB4 – Plakin domain fragment of Human plectin (C-terminal region spectrin repeats: SR7-SR8-SR9) Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR7-SR8-SR9 monomer, 43 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 4.2 nm Dmax 17.0 nm VolumePorod 57 nm3

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9) Sample: Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 monomer, 96 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 13 RgGuinier 8.5 nm Dmax 35.0 nm VolumePorod 135 nm3

SASDBD4 – Plakin domain fragment of Human desmoplakin (C-terminal region spectrin repeats: SR7-SR8-SR9) Sample: Plakin domain fragment of Human Desmoplakin encompassing spectrin repeats SR7-SR8-SR9 monomer, 42 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25 RgGuinier 4.4 nm Dmax 17.5 nm VolumePorod 69 nm3

SASDBE4 – Plakin domain of Human desmoplakin Sample: Plakin domain of Human Desmoplakin monomer, 99 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 25 RgGuinier 7.5 nm Dmax 35.0 nm VolumePorod 136 nm3