Global shape of SvGT, a metal-dependent bacteriocin modifying S/O-HexNActransferase from actinobacteria: c -terminal dimerization modulates the function of this GT.

Sharma Y, Ahlawat S, Ashish, Rao A
J Biomol Struct Dyn :1-15 (2023 Sep 15)
PMID: 37712855
doi: 10.1080/07391102.2023.2255906 		Submitted to SASBDB: 2022 Mar 23
Published in SASBDB:

SASDN98 – SvGT under calcium free conditions: Apo state

Glyco_trans_2-like domain-containing protein experimental SAS data
OTHER model
Sample: Glyco_trans_2-like domain-containing protein dimer, 96 kDa Streptomyces sp. M41(2017) protein
Buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, and 2 mM DTT + 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 18
RgGuinier 4.4 nm
Dmax 17.0 nm
VolumePorod 151 nm3

SASDNA8 – SvGT under calcium free conditions: Mg2+ bound state

Glyco_trans_2-like domain-containing protein experimental SAS data
OTHER model
Sample: Glyco_trans_2-like domain-containing protein dimer, 96 kDa Streptomyces sp. M41(2017) protein
Buffer: 20 mM Tris, pH 7.5, 100 mM NaCl, 2 mM DTT, 1 mM EDTA, 2 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2019 Oct 18
RgGuinier 5.8 nm
Dmax 17.9 nm
VolumePorod 111 nm3