Solution structural studies of pre-amyloid oligomer states of the biofilm protein Aap.

Yarawsky AE, Hopkins JB, Chatzimagas L, Hub JS, Herr AB
J Mol Biol :167708 (2022 Jun 28)

PMID: 35777467 doi: 10.1016/j.jmb.2022.167708

Submitted to SASBDB: 2022 May 9 Published in SASBDB: 2022 Jul 4

SASDP43 – Accumulation-association protein (Aap) Brpt5.5 monomer Sample: Accumulation associated protein monomer, 78 kDa Staphylococcus epidermidis (strain … protein Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Oct 16 RgGuinier 14.7 nm Dmax 55.8 nm

SASDP53 – Accumulation-association protein (Aap) Brpt5.5 5xH85A monomer Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1 RgGuinier 14.1 nm Dmax 57.2 nm

SASDP63 – Accumulation-association protein (Aap) Brpt5.5 2xH85A monomer Sample: Accumulation associated protein (mutant) monomer, 78 kDa Staphylococcus epidermidis (strain … protein Buffer: 50 mM MOPS, 50 mM NaCl, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1 RgGuinier 14.4 nm Dmax 56.6 nm

SASDP73 – Accumulation-association protein (Aap) Brpt5.5 5xH85A dimer in the presence of ZnCl2 Sample: Accumulation associated protein (mutant) dimer, 155 kDa Staphylococcus epidermidis (strain … protein Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1 RgGuinier 15.0 nm Dmax 63.3 nm

SASDP83 – Accumulation-association protein (Aap) Brpt5.5 2xH85A tetramer in the presence of ZnCl2 Sample: Accumulation associated protein (mutant) tetramer, 312 kDa Staphylococcus epidermidis (strain … protein Buffer: 50 mM MOPS, 50 mM NaCl, 5 mM ZnCl2, pH: 7.2 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Mar 1 RgGuinier 15.7 nm Dmax 70.0 nm