Mechanistic insights into enhancement or inhibition of phase separation by different polyubiquitin chains.

Dao TP, Yang Y, Presti MF, Cosgrove MS, Hopkins JB, Ma W, Loh SN, Castañeda CA
EMBO Rep :e55056 (2022 Jun 28)

PMID: 35762418 doi: 10.15252/embr.202255056

Submitted to SASBDB: 2022 May 11 Published in SASBDB: 2022 Jul 4

SASDP93 – K48-linked tetraubiquitin (Ub4) Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8 RgGuinier 2.6 nm Dmax 95.0 nm VolumePorod 44 nm3

SASDPA3 – K63-linked tetraubiquitin (Ub4) Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8 RgGuinier 3.2 nm Dmax 13.0 nm VolumePorod 44 nm3

SASDPB3 – M1-linked tetraubiquitin (Ub4) Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8 RgGuinier 3.2 nm Dmax 12.0 nm VolumePorod 46 nm3

SASDPC3 – HOTag6 tetramerization domain followed by a 10-Gly linker and monoubiquitin Sample: Polyubiquitin-B tetramer, 53 kDa Homo sapiens protein Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8 Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Oct 8 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 115 nm3