Argonaute protein SAXS investigation

lirong zheng Submitted to SASBDB: 2022 May 15
Published in SASBDB:

SASDNE8 – apo-Pyrococcus furiosus argonaute protein at 10 °C

Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 142 nm3

SASDPM2 – apo-Pyrococcus furiosus argonaute protein at 25 °C

Piwi domain-containing protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 2.9 nm
Dmax 9.7 nm
VolumePorod 142 nm3

SASDPN2 – apo-Pyrococcus furiosus argonaute protein at 95 °C

Piwi domain-containing protein experimental SAS data
GASBOR model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 187 nm3

SASDPP2 – apo-Pyrococcus furiosus argonaute protein with urea at 37 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 91 kDa Pyrococcus furiosus (strain … protein
Buffer: 20 mM Tris-HCl, 250 mM NaCl, 2 mM DTT, 6 M urea, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 Nov 17
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 148 nm3

SASDPQ2 – apo-Clostridium butyricum argonaute protein at 10 °C

Clostridium butyricum argonaute protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Clostridium butyricum argonaute protein monomer, 86 kDa protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 188 nm3

SASDPR2 – apo-Clostridium butyricum argonaute protein at 37 °C

Clostridium butyricum argonaute protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Clostridium butyricum argonaute protein monomer, 86 kDa protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.2 nm
Dmax 9.7 nm
VolumePorod 185 nm3

SASDPS2 – apo-Thermus thermophilus argonaute protein at 10 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 122 nm3

SASDPT2 – apo-Thermus thermophilus argonaute protein at 25 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.1 nm
Dmax 9.7 nm
VolumePorod 120 nm3

SASDPU2 – apo-Thermus thermophilus argonaute protein at 75 °C

Piwi domain-containing protein experimental SAS data
MOLECULAR DYNAMICS FRAME model
Sample: Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer: 20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7
RgGuinier 3.3 nm
Dmax 9.7 nm
VolumePorod 175 nm3