A solution structure analysis reveals a bent collagen triple helix in the complement activation recognition molecule mannan-binding lectin.

Iqbal H, Fung KW, Gor J, Bishop AC, Makhatadze GI, Brodsky B, Perkins SJ
J Biol Chem 299(2):102799 (2023 Feb)

PMID: 36528062 doi: 10.1016/j.jbc.2022.102799

Submitted to SASBDB: 2023 Jan 11 Published in SASBDB: 2023 Dec 7

SASDRS2 – Collagen-like peptide Mannan-binding lectin (MBL) center Sample: Ac-(POG)4-QG-(POG)5-NH2 trimer, 11 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.1 nm Dmax 8.7 nm VolumePorod 7 nm3

SASDRT2 – Collagen-like peptide Mannan-binding Lectin (MBL) native Sample: Ac-(POG)4-QG-(POG)5-NH2 trimer, 11 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 1.7 nm Dmax 7.1 nm VolumePorod 4 nm3

SASDRU2 – Collagen-like peptide Mannan-binding lectin (MBL) with EOG sequence Sample: Ac-(POG)5-EOGQGLRG-(POG)3-NH2 trimer, 12 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.3 nm Dmax 10.0 nm VolumePorod 6 nm3

SASDRV2 – Collagen-like peptide Mannan-binding lectin (MBL) 12 triplets Sample: Ac-(POG)5-EPGQGLRG-(POG)5-NH2 trimer, 14 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.8 nm Dmax 12.5 nm VolumePorod 16 nm3

SASDRW2 – [(Pro-Hyp-Gly)10]3 Sample: Ac-(POG)10-NH2 trimer, 12 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.2 nm Dmax 8.5 nm VolumePorod 7 nm3

SASDRX2 – [(Pro-Hyp-Gly)13]3 Sample: Ac-(POG)13-NH2 trimer, 15 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.8 nm Dmax 9.5 nm VolumePorod 14 nm3

SASDRY2 – [(Pro-Pro-Gly)10]3 Sample: [(Pro-Pro-Gly)10]3 trimer, 8 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.3 nm Dmax 9.7 nm VolumePorod 9 nm3

SASDRZ2 – [(Pro-Hyp-Gly)4-POA-(Pro-Hyp-Gly)5]3 Sample: Ac-(POG)4-POA-(POG)5-NH2 trimer, 12 kDa protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.3 nm Dmax 11.0 nm VolumePorod 10 nm3

SASDR23 – [(POG)3-ITGARGLAG-(POG)4]3 Sample: Ac-(POG)3-ITGARGLAG-(POG)4-NH2 trimer, 11 kDa synthetic construct protein Buffer: 20 mM L-histidine, 138 mM NaCl, 2.7 mM KCL,, pH: 6 Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Feb 1 RgGuinier 2.2 nm Dmax 10.0 nm VolumePorod 9 nm3