SEC-SAXS: Experimental set-up and software developments build up a powerful tool.

PĂ©rez J, Thureau A, Vachette P
Methods Enzymol 677:221-249 (2022)
PMID: 36410950
doi: 10.1016/bs.mie.2022.08.024
Submitted to SASBDB: 2022 May 23
Published in SASBDB:

SASDPH3 – Benchmarking extraction methods of scattering patterns of poorly resolved components from a SEC-SAXS dataset of BSA.

Bovine Serum Albumin experimental SAS data
Bovine Serum Albumin Kratky plot
Sample: Bovine Serum Albumin trimer, 199 kDa Bos taurus protein
Buffer: 20 mM Hepes, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Sep 24
RgGuinier 5.2 nm
Dmax 17.6 nm
VolumePorod 370 nm3